|
| 1 | +# coding=utf-8 |
| 2 | +# Copyright 2023 The Google Research Authors. |
| 3 | +# |
| 4 | +# Licensed under the Apache License, Version 2.0 (the "License"); |
| 5 | +# you may not use this file except in compliance with the License. |
| 6 | +# You may obtain a copy of the License at |
| 7 | +# |
| 8 | +# http://www.apache.org/licenses/LICENSE-2.0 |
| 9 | +# |
| 10 | +# Unless required by applicable law or agreed to in writing, software |
| 11 | +# distributed under the License is distributed on an "AS IS" BASIS, |
| 12 | +# WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied. |
| 13 | +# See the License for the specific language governing permissions and |
| 14 | +# limitations under the License. |
| 15 | +"""Affine transform decorators.""" |
| 16 | + |
| 17 | +from typing import Any, Mapping, MutableMapping, Optional, Sequence |
| 18 | + |
| 19 | +from connectomics.common import opencv_utils |
| 20 | +from connectomics.volume.decorators import Decorator # pylint: disable=g-importing-member |
| 21 | +import gin |
| 22 | +import numpy as np |
| 23 | +import skimage.feature |
| 24 | +import tensorstore as ts |
| 25 | + |
| 26 | +JsonSpec = Mapping[str, Any] |
| 27 | +MutableJsonSpec = MutableMapping[str, Any] |
| 28 | + |
| 29 | + |
| 30 | +@gin.register |
| 31 | +class OptimAffineTransformSectionwise(Decorator): |
| 32 | + """Finds 2D affine transforms sectionwise by ECC optimization.""" |
| 33 | + |
| 34 | + def __init__(self, |
| 35 | + fixed_spec: JsonSpec, |
| 36 | + image_dims: Sequence[str] = ('x', 'y'), |
| 37 | + batch_dim: Optional[str] = None, |
| 38 | + init_previous: bool = False, |
| 39 | + context_spec: Optional[MutableJsonSpec] = None, |
| 40 | + **optim_args): |
| 41 | + """Optimize affine transform sectionwise. |
| 42 | +
|
| 43 | + Uses OpenCV's `cv.findTransformECC` to find an affine transformations that |
| 44 | + aligns moving and fixed 2D images, where moving images are taken from the |
| 45 | + input TensorStore and fixed images from the one specified by `fixed_spec`. |
| 46 | +
|
| 47 | + Note that optimisation is done per 2D section according to `image_dims`. |
| 48 | + The resulting TensorStore contains 2x3 transformation matrices in |
| 49 | + dimensions 'r' (row) and 'c' (column), for all non-image dimensions. |
| 50 | + Transformation matrices are stored in individual chunks. |
| 51 | +
|
| 52 | + Args: |
| 53 | + fixed_spec: TensorStore containing fixed images to align against. |
| 54 | + Must have same dimensions as input TS (labels and shape). |
| 55 | + image_dims: Image dimensions to transform, e.g., `x` and `y` (two). |
| 56 | + batch_dim: Optional dimension to batch reads. |
| 57 | + init_previous: If True, initializes transform for subsequent calls |
| 58 | + of the optimization function with the previous result. Requires |
| 59 | + specification of a `batch_dim`. |
| 60 | + context_spec: Spec for virtual chunked context overriding its defaults. |
| 61 | + **optim_args: Passed to `opencv_utils.optim_transform`. |
| 62 | + """ |
| 63 | + super().__init__(context_spec) |
| 64 | + self._fixed_spec = fixed_spec |
| 65 | + self._image_dims = image_dims |
| 66 | + self._batch_dim = batch_dim |
| 67 | + self._init_previous = init_previous |
| 68 | + if init_previous and not batch_dim: |
| 69 | + raise ValueError('`batch_dim` must be specified to use `init_previous`.') |
| 70 | + if 'transform_initial' in optim_args: |
| 71 | + self._transform_initial = optim_args['transform_initial'] |
| 72 | + optim_args.pop('transform_initial') |
| 73 | + else: |
| 74 | + self._transform_initial = None |
| 75 | + self._optim_args = optim_args |
| 76 | + |
| 77 | + def decorate(self, input_ts: ts.TensorStore) -> ts.TensorStore: |
| 78 | + """Wraps input TensorStore with a virtual_chunked for optim_transform.""" |
| 79 | + |
| 80 | + fixed_ts = ts.open(self._fixed_spec).result() |
| 81 | + if input_ts.domain.labels != fixed_ts.domain.labels: |
| 82 | + raise ValueError( |
| 83 | + 'Input TS and fixed TS must have same labels, but they are ' + |
| 84 | + f'{input_ts.domain.labels} and {fixed_ts.domain.labels}.') |
| 85 | + if input_ts.shape != fixed_ts.shape: |
| 86 | + raise ValueError( |
| 87 | + 'Input TS and fixed TS must have same shape, but they are ' + |
| 88 | + f'{input_ts.shape} and {fixed_ts.shape}.') |
| 89 | + |
| 90 | + if len(self._image_dims) != 2: |
| 91 | + raise ValueError( |
| 92 | + f'2 image dimensions are required, but got {len(self._image_dims)}.') |
| 93 | + for d in self._image_dims: |
| 94 | + if d not in input_ts.domain.labels: |
| 95 | + raise ValueError( |
| 96 | + f'image dimension {d} not among labels {input_ts.domain.labels}.') |
| 97 | + elif input_ts.domain[d].size < 2: |
| 98 | + raise ValueError( |
| 99 | + 'image dimension {d} must at least have size 2 but has size: ' + |
| 100 | + f'{input_ts.domain[d].size}.') |
| 101 | + |
| 102 | + non_image_dims = [ |
| 103 | + l for l in input_ts.domain.labels if l not in self._image_dims] |
| 104 | + input_domain_dict = {dim.label: dim for dim in list(input_ts.domain)} |
| 105 | + batch_idx = (input_ts.domain.labels.index(self._batch_dim) |
| 106 | + if self._batch_dim else None) |
| 107 | + |
| 108 | + def read_fn(domain: ts.IndexDomain, array: np.ndarray, |
| 109 | + unused_read_params: ts.VirtualChunkedReadParameters): |
| 110 | + domain_dict = {dim.label: dim for dim in list(domain)} |
| 111 | + |
| 112 | + if self._transform_initial: |
| 113 | + transform_initial = self._transform_initial.copy() |
| 114 | + else: |
| 115 | + transform_initial = None |
| 116 | + |
| 117 | + if not self._batch_dim: |
| 118 | + read_domain = [] |
| 119 | + for l in non_image_dims: |
| 120 | + read_domain.append(domain_dict[l]) |
| 121 | + for l in self._image_dims: |
| 122 | + read_domain.append(input_domain_dict[l]) |
| 123 | + read_domain = ts.IndexDomain(read_domain) |
| 124 | + |
| 125 | + # Images are transposed since OpenCV uses [x,y]-convention. |
| 126 | + # See `opencv_utils` for more details. |
| 127 | + _, transform = opencv_utils.optim_transform( |
| 128 | + fix=np.array(fixed_ts[read_domain], dtype=np.float32).squeeze().T, |
| 129 | + mov=np.array(input_ts[read_domain], dtype=np.float32).squeeze().T, |
| 130 | + transform_initial=transform_initial, |
| 131 | + **self._optim_args) |
| 132 | + |
| 133 | + array[...] = transform.reshape(array.shape) |
| 134 | + else: |
| 135 | + for i, j in enumerate(domain_dict[self._batch_dim]): |
| 136 | + read_domain = [] |
| 137 | + for l in non_image_dims: |
| 138 | + if l != self._batch_dim: |
| 139 | + read_domain.append(domain_dict[l]) |
| 140 | + else: |
| 141 | + read_domain.append( |
| 142 | + ts.Dim(inclusive_min=j, exclusive_max=j+1, |
| 143 | + label=self._batch_dim)) |
| 144 | + for l in self._image_dims: |
| 145 | + read_domain.append(input_domain_dict[l]) |
| 146 | + read_domain = ts.IndexDomain(read_domain) |
| 147 | + |
| 148 | + # Images are transposed since OpenCV uses [x,y]-convention. |
| 149 | + # See `opencv_utils` for more details. |
| 150 | + _, transform = opencv_utils.optim_transform( |
| 151 | + fix=np.array(fixed_ts[read_domain], dtype=np.float32).squeeze().T, |
| 152 | + mov=np.array(input_ts[read_domain], dtype=np.float32).squeeze().T, |
| 153 | + transform_initial=transform_initial, |
| 154 | + **self._optim_args) |
| 155 | + if self._init_previous: |
| 156 | + transform_initial = transform |
| 157 | + |
| 158 | + idx = [slice(None) for _ in range(array.ndim)] |
| 159 | + idx[batch_idx] = i |
| 160 | + array[tuple(idx)] = transform.reshape(array[tuple(idx)].shape) |
| 161 | + |
| 162 | + chunksize = [2, 3] |
| 163 | + for l in non_image_dims: |
| 164 | + if l != self._batch_dim: |
| 165 | + chunksize.append(1) |
| 166 | + else: |
| 167 | + chunksize.append(input_domain_dict[l].size) |
| 168 | + schema = { |
| 169 | + 'chunk_layout': { |
| 170 | + 'read_chunk': {'shape': chunksize}, |
| 171 | + 'write_chunk': {'shape': chunksize}, |
| 172 | + }, |
| 173 | + 'domain': { |
| 174 | + 'labels': ['r', 'c',] + non_image_dims, |
| 175 | + 'inclusive_min': [0, 0] + [ |
| 176 | + input_domain_dict[l].inclusive_min for l in non_image_dims], |
| 177 | + 'exclusive_max': [2, 3] + [ |
| 178 | + input_domain_dict[l].exclusive_max for l in non_image_dims], |
| 179 | + }, |
| 180 | + 'dtype': 'float64', |
| 181 | + 'rank': len(chunksize), |
| 182 | + } |
| 183 | + |
| 184 | + return ts.virtual_chunked( |
| 185 | + read_fn, schema=ts.Schema(schema), context=self._context) |
| 186 | + |
| 187 | + |
| 188 | +@gin.register |
| 189 | +class OptimTranslationTransform(Decorator): |
| 190 | + """Finds 2D/3D translations for registration via cross-correlation.""" |
| 191 | + |
| 192 | + def __init__(self, |
| 193 | + fixed_spec: JsonSpec, |
| 194 | + image_dims: Sequence[str] = ('x', 'y'), |
| 195 | + context_spec: Optional[MutableJsonSpec] = None, |
| 196 | + **optim_args): |
| 197 | + """Computes cross-correlation between volumes for registration. |
| 198 | +
|
| 199 | + Uses skimage's `registration.phase_cross_correlation` to find translation |
| 200 | + matrices for registration of two volumes, where 2D or 3D moving images are |
| 201 | + taken from the input TensorStore and fixed images from the one specified by |
| 202 | + `fixed_spec`. |
| 203 | +
|
| 204 | + The resulting TensorStore contains 2x3 (2D) or 3x4 (3D) transformation |
| 205 | + matrices in dimensions 'r' (row) and 'c' (column), for all non-image |
| 206 | + dimensions. Transformation matrices are stored in individual chunks. |
| 207 | +
|
| 208 | + Args: |
| 209 | + fixed_spec: TensorStore containing fixed images to align against. |
| 210 | + Must have same dimensions as input TS (labels and shape). |
| 211 | + image_dims: Image dimensions to transform, e.g., `x` and `y` (two). |
| 212 | + context_spec: Spec for virtual chunked context overriding its defaults. |
| 213 | + **optim_args: Passed to `skimage.registration.phase_cross_correlation`. |
| 214 | + """ |
| 215 | + super().__init__(context_spec) |
| 216 | + self._fixed_spec = fixed_spec |
| 217 | + self._image_dims = image_dims |
| 218 | + self._optim_args = optim_args |
| 219 | + |
| 220 | + def decorate(self, input_ts: ts.TensorStore) -> ts.TensorStore: |
| 221 | + """Wraps input TensorStore with a virtual_chunked.""" |
| 222 | + |
| 223 | + fixed_ts = ts.open(self._fixed_spec).result() |
| 224 | + if input_ts.domain.labels != fixed_ts.domain.labels: |
| 225 | + raise ValueError( |
| 226 | + 'Input TS and fixed TS must have same labels, but they are ' + |
| 227 | + f'{input_ts.domain.labels} and {fixed_ts.domain.labels}.') |
| 228 | + if input_ts.shape != fixed_ts.shape: |
| 229 | + raise ValueError( |
| 230 | + 'Input TS and fixed TS must have same shape, but they are ' + |
| 231 | + f'{input_ts.shape} and {fixed_ts.shape}.') |
| 232 | + |
| 233 | + num_image_dims = len(self._image_dims) |
| 234 | + if num_image_dims not in (2, 3): |
| 235 | + raise ValueError( |
| 236 | + f'2 or 3 image dimensions are required, but got {num_image_dims}.') |
| 237 | + for d in self._image_dims: |
| 238 | + if d not in input_ts.domain.labels: |
| 239 | + raise ValueError( |
| 240 | + f'image dimension {d} not among labels {input_ts.domain.labels}.') |
| 241 | + elif input_ts.domain[d].size < 2: |
| 242 | + raise ValueError( |
| 243 | + 'image dimension {d} must at least have size 2 but has size: ' + |
| 244 | + f'{input_ts.domain[d].size}.') |
| 245 | + |
| 246 | + non_image_dims = [ |
| 247 | + l for l in input_ts.domain.labels if l not in self._image_dims] |
| 248 | + input_domain_dict = {dim.label: dim for dim in list(input_ts.domain)} |
| 249 | + |
| 250 | + def read_fn(domain: ts.IndexDomain, array: np.ndarray, |
| 251 | + unused_read_params: ts.VirtualChunkedReadParameters): |
| 252 | + domain_dict = {dim.label: dim for dim in list(domain)} |
| 253 | + |
| 254 | + read_domain = [] |
| 255 | + for l in non_image_dims: |
| 256 | + read_domain.append(domain_dict[l]) |
| 257 | + for l in self._image_dims: |
| 258 | + read_domain.append(input_domain_dict[l]) |
| 259 | + read_domain = ts.IndexDomain(read_domain) |
| 260 | + |
| 261 | + # Default to no normalization. |
| 262 | + if 'normalization' not in self._optim_args: |
| 263 | + self._optim_args['normalization'] = None |
| 264 | + |
| 265 | + translation, _, _ = skimage.registration.phase_cross_correlation( |
| 266 | + reference_image=np.array( |
| 267 | + fixed_ts[read_domain], dtype=np.float32).squeeze(), |
| 268 | + moving_image=np.array( |
| 269 | + input_ts[read_domain], dtype=np.float32).squeeze(), |
| 270 | + **self._optim_args) |
| 271 | + transform = np.hstack( |
| 272 | + (np.eye(len(self._image_dims)), translation.reshape(-1, 1))) |
| 273 | + |
| 274 | + array[...] = transform.reshape(array.shape) |
| 275 | + |
| 276 | + chunksize = [num_image_dims, num_image_dims + 1] |
| 277 | + for _ in non_image_dims: |
| 278 | + chunksize.append(1) |
| 279 | + schema = { |
| 280 | + 'chunk_layout': { |
| 281 | + 'read_chunk': {'shape': chunksize}, |
| 282 | + 'write_chunk': {'shape': chunksize}, |
| 283 | + }, |
| 284 | + 'domain': { |
| 285 | + 'labels': ['r', 'c',] + non_image_dims, |
| 286 | + 'inclusive_min': [0, 0] + [ |
| 287 | + input_domain_dict[l].inclusive_min for l in non_image_dims], |
| 288 | + 'exclusive_max': [num_image_dims, num_image_dims + 1] + [ |
| 289 | + input_domain_dict[l].exclusive_max for l in non_image_dims], |
| 290 | + }, |
| 291 | + 'dtype': 'float64', |
| 292 | + 'rank': len(chunksize), |
| 293 | + } |
| 294 | + |
| 295 | + return ts.virtual_chunked( |
| 296 | + read_fn, schema=ts.Schema(schema), context=self._context) |
0 commit comments