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Stocks and Buffers

Some commonly stock buffers used in the lab are. Expand as you learn more about the workflow of the user:

  • 1 M HEPES pH 7.4 @ 20 °C
  • 5 M NaCl
  • 3 M KCl
  • 1 M MgCl2
  • 2 M Tris-HCl (pH depending on context)
  • 50 % (v/v) glycerol
  • 2 M imidazole pH 8.0
  • 0.5 M TCEP pH 7.5
  • 1 M DTT
  • 1000X Ampicillin
  • 1000X Carbenicllin
  • 1000X Chloramphenicol
  • 1000X Kanamycin
  • 0.5 M EDTA pH 8.0