Generate New Reference Genome: ERROR: More than one record found in handle

[bfurtwa]

I put all variants in a new variant set and generated a new reference genome using this variant set. When I click submit, I get the error: "ERROR: More than one record found in handle"

[glebkuznetsov]

Does your original reference genome have multiple records? Are you able to share it? Thanks!

[bfurtwa]

I used NZ_CP009974 and the plasmid NZ_CP009975 in one .gb file produced by geneious 8

[glebkuznetsov]

Got it. It's likely we didn't test the case of generating a new reference genome for the combined genome/plasmid case, but the fix may be straightforward. Do you mind attaching the combined .gb file?

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On Thu, Mar 8, 2018 at 5:45 AM Benjamin Furtwängler < ***@***.***> wrote: I used NZ_CP009974 and the plasmid NZ_CP009975 in one .gb file produced by geneious 8 — You are receiving this because you commented. Reply to this email directly, view it on GitHub <#699 (comment)>, or mute the thread <https://github.com/notifications/unsubscribe-auth/AAORu1tDMRJk64ZHmnvSwPqp0BvxfeHAks5tcQhSgaJpZM4Sg51X> .

[bfurtwa]

NZ_CP009975_4.zip

[glebkuznetsov]

Thanks. We'll look into fixing this issue with versioning reference genomes with multiple records (e.g. w/ plasmid). In the meantime, if you need the versioned genomed, you may consider aligning to the reference with NZ_CP009974 only.

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On Thu, Mar 8, 2018 at 8:49 AM Benjamin Furtwängler < ***@***.***> wrote: NZ_CP009975_4.zip <https://github.com/churchlab/millstone/files/1793368/NZ_CP009975_4.zip> — You are receiving this because you commented. Reply to this email directly, view it on GitHub <#699 (comment)>, or mute the thread <https://github.com/notifications/unsubscribe-auth/AAORu9fSLuI91StQx3w0PtEDJu7Pc6VIks5tcTbTgaJpZM4Sg51X> .