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SIPEC
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=====
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|Code style: black|
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SIPEC: the deep-learning Swiss knife for behavioral data analysis
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This is the repository accompanying the `SIPEC
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publication\* <https://doi.org/10.1101/2020.10.26.355115>`__, which is a
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pipeline that enables all-round behavioral analysis through the usage of
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state-of-the-art neural networks. You can use SIPEC by either combining
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its modules in your own workflow, or using template workflows, that have
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been used in the paper, which can be accessed via command line. We will
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be providing more detailed and illustrated instructions soon. Moreover,
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extensive documentation and more exemplary data will be made available.
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We welcome feedback via GitHub issues.
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- Markus Marks, Jin Qiuhan, Oliver Sturman, Lukas von Ziegler, Sepp
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Kollmorgen, Wolfger von der Behrens, Valerio Mante, Johannes Bohacek,
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Mehmet Fatih Yanik bioRxiv 2020.10.26.355115; doi:
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https://doi.org/10.1101/2020.10.26.355115
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|image1|
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Usage/Installation
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------------------
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**For really making use of SIPEC, your machine should have a powerful
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GPU. We have tested the scripts with NVIDIA GTX 1080, NVIDIA GTX 2080 Ti
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and V100 GPUs.**
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**For using SIPEC, your machine should have a powerful GPU. We have
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tested the scripts with NVIDIA GTX 1080, NVIDIA GTX 2080 Ti and V100
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GPUs.**
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Docker
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~~~~~~
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::
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docker pull chadhat/sipec:tf2
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docker pull sipec/sipec:latest
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**Note:** In order to run docker without ``sudo`` you would need to
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create a docker group and add your user to it. Please follow the
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instructions on:
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https://docs.docker.com/engine/install/linux-postinstall/
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The docker image contains the environment and SIPEC scripts.
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The docker image contains the environment, sample data and SIPEC
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scripts.
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Environment installation
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~~~~~~~~~~~~~~~~~~~~~~~~
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If you do not want to use the docker container you can follow these
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installation instructions for **Linux**. These instructions have been
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tested on Ubuntu 18 and 20.04.
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tested on Ubuntu 20.04 but would most likely also work on Ubuntu 18.
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Step 1: Install Cuda 11.0.3
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^^^^^^^^^^^^^^^^^^^^^^^^^^^
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Download and install Cuda 11. We have tested the setup with cuda 11.0.3.
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Download and install Cuda 11.0.3 (We have tested the setup with this
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cuda version).
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After the installation is finised run ``nvcc --version`` to check the
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installed cuda version.
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The script will ask you for the root password.
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Step 4:
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^^^^^^^
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The script ``setup.sh`` has created a virtual environment named ``env``
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in the repository folder. Activate the environment by executing:
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::
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source ./env/bin/activate
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Step 5:
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^^^^^^^
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To test your setup run one of the scripts in the folder ``SwissKnife``,
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e.g.,
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::
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python segmentation.py --help
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Usage
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-----
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.. raw:: html
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<pre><code>
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docker container run -v "<b>RESULTS_PATH</b>:/home/user/results" --runtime=nvidia --rm sipec:main_tf2
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docker run -v "<b>RESULTS_PATH</b>:/home/user/results" --runtime=nvidia --rm sipec/sipec
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segmentation.py --cv_folds 0 --gpu 0 --frames /home/user/data/mouse_segmentation_4plex_merged/frames --annotations /home/user/data/mouse_segmentation_4plex_merged/merged.json
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docker run -v "<b>RESULTS_PATH</b>:/home/user/results" --runtime=nvidia --rm sipec/sipec
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classification_comparison.py --gpu 0 --config_name behavior_config_final --random_seed 1 --output_path=/home/user/results
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docker container run -v "<b>RESULTS_PATH</b>:/home/user/results" --runtime=nvidia --rm sipec:main_tf2
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poseestimation.py --gpu 0 --operation train_mouse --output_path=/home/user/results/
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docker run -v "<b>RESULTS_PATH</b>:/home/user/results" --runtime=nvidia --rm sipec/sipec
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poseestimation.py --gpu 0 --results_sink /home/user/results --dlc_path /home/user/data/mouse_pose/OFT/labeled-data/ --segnet_path /home/user/data/pretrained_networks/mask_rcnn_mouse_0095.h5 --config poseestimation_config_test
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docker container run -v "<b>RESULTS_PATH</b>:/home/user/results" --runtime=nvidia --rm sipec:main_tf2
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behavior.py --gpu 0 --annotations /home/user/data/20180124T113800-20180124T115800_0.csv --video /home/user/data/fullvids_20180124T113800-20180124T115800_%T1_0.mp4 --output_path /home/user/results
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docker run -v "<b>RESULTS_PATH</b>:/home/user/results" --runtime=nvidia --rm sipec/sipec
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full_inference.py --gpu 0 --species mouse --video /home/user/data/full_inference_posenet_25_June/animal1234_day1.avi --posenet_path /home/user/data/pretrained_networks/posenet_mouse.h5 --segnet_path /home/user/data/pretrained_networks/mask_rcnn_mouse_0095.h5 --max_ids 4 --results_sink /home/user/results/full_inference
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docker container run -v "<b>RESULTS_PATH</b>:/home/user/results" --runtime=nvidia --rm sipec:main_tf2
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full_inference.py --gpu 0 --species mouse --video /home/user/data/full_inference_and_vis_data/animal5678_day2.avi --segnet_path "/home/user/data/full_inference_and_vis_data/mask_rcnn_mouse_0095.h5" --max_ids 4 --results_sink /home/user/results/full_inference
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<b>Coming soon</b>: behavior.py
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docker container run -v "<b>RESULTS_PATH</b>:/home/user/results" --runtime=nvidia --rm sipec:main_tf2
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segmentation.py --cv_folds 0 --gpu 0 --frames /home/user/data/mouse_segmentation_single/annotated_frames --annotations /home/user/data/mouse_segmentation_single/mouse_top_segmentation.json
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</pre>
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Where, **RESULTS_PATH** is the path on your machine where you would like
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::
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docker container run --runtime=nvidia --rm sipec:main_tf2 segmentation.py --help
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docker container run --runtime=nvidia --rm sipec/sipec segmentation.py --help
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own pipline
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~~~~~~~~~~~
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------------
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Mouse OFT behavioral videos
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~~~~~~~~~~~~~~~~~~~~~~~~~~~
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'''''''''''''''''''''''''''
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For open field (OFT) mouse behavioral analysis, you can use the
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exemplary data from Sturman et al. from zenedo.
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https://zenodo.org/record/3608658 The corresponding labels can be
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accessed here.
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https://github.com/ETHZ-INS/DLCAnalyzer/tree/master/data/OFT/Labels
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Primate, Mouse data for different SIPEC modules (incomplete, being updated at the moment)
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'''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''
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https://www.dropbox.com/sh/dpkswv0j3l3j38r/AABwHUdL6XYvrhDLDSlyPFzZa?dl=0
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Pretrained Networks
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'''''''''''''''''''
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https://www.dropbox.com/sh/y387kik9mwuszl3/AABBVWALEimW-hrbXvdfjHQSa?dl=0
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Cite
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----
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SIPEC: the deep-learning Swiss knife for behavioral data analysis
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Markus Marks, Jin Qiuhan, Oliver Sturman, Lukas von Ziegler, Sepp Kollmorgen, Wolfger von der Behrens, Valerio Mante, Johannes Bohacek, Mehmet Fatih Yanik
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bioRxiv 2020.10.26.355115; doi: https://doi.org/10.1101/2020.10.26.355115
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.. |Code style: black| image:: https://img.shields.io/badge/code%20style-black-000000.svg
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:target: https://github.com/psf/black
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.. |image1| image:: supp_files/Supplementary%20Video%201.gif
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